Donald, T.Leach, C.Clough, A.Timmis, J.2006-07-052006-07-051995Heredity, 1995; 74(5):556-5610018-067X1365-2540http://hdl.handle.net/2440/11457Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from 0B and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.enChromosomesPlantsRNA, PlantRNA, RibosomalBlotting, SouthernIn Situ Hybridization, FluorescenceMethylationJournal article0030004296001995169810.1038/hdy.1995.77A1995QX259000122-s2.0-002929468868302