Yun, Q.Chen, T.Zhang, G.Bi, J.Ma, G.Su, Z.2008-07-142008-07-142005Biotechnology Letters, 2005; 27(3):213-2170141-54921573-6776http://hdl.handle.net/2440/46419The definitive version may be found at www.springerlink.comA novel methoxypolyethylene glycol (mPEG) derivative, containing a reactive group of 1-methyl pyridinium toluene-4-sulfonate, was synthesized and characterized. The mPEG derivative was successfully conjugated with two proteins: recombinant human granulocyte-colony stimulating factor (rhG-CSF) and consensus interferon (C-IFN). Homogeneous mono-PEGylated proteins were obtained which were identified by high performance size-exclusion chromatography and MALDI-TOF mass spectrometry. The biological activities of the mono-PEGylated rhG-CSF and the mono-PEGylated C-IFN were maintained at 90% and 88%, respectively.en© Springerconsensus interferongranulocyte-colony stimulating factorPEGylationpolyethylene glycolJournal article002007782710.1007/s10529-004-8354-70002271611000122-s2.0-1764439639744003Bi, J. [0000-0001-7056-8572]