Kosik, O.Auburn, R.Russell, S.Stratilova, E.Garajova, S.Hrmova, M.Farkas, V.2010-09-212010-09-212010Glycoconjugate Journal, 2010; 27(1):79-870282-00801573-4986http://hdl.handle.net/2440/60683Polysaccharide transglycosylases catalyze disproportionation of polysaccharide molecules by cleaving glycosidic linkages in polysaccharide chains and transferring their cleaved portions to hydroxyl groups at the non-reducing ends of other polysaccharide or oligosaccharide molecules. In plant cell walls, transglycosylases have a potential to catalyze both cross-linking of polysaccharide molecules and grafting of newly arriving polysaccharide molecules into the cell wall structure during cell growth. Here we describe a polysaccharide microarray in form of a glycochip permitting simultaneous high-throughput monitoring of multiple transglycosylase activities in plant extracts. The glycochip, containing donor polysaccharides printed onto nitrocellulose-coated glass slides, was incubated with crude plant extracts, along with a series of fluorophore-labelled acceptor oligosaccharides. After removing unused labelled oligosaccharides by washing, fluorescence retained on the glycochip as a result of transglycosylase reaction was detected with a standard microarray scanner. The glycochip assay was used to detect transglycosylase activities in crude extracts from nasturtium (Tropaeolum majus) and mouse-ear cress (Arabidopsis thaliana). A number of previously unknown saccharide donor-acceptor pairs active in transglycosylation reactions that lead to the formation of homo- and hetero-glycosidic conjugates, were detected. Our data provide experimental support for the existence of diverse transglycosylase activities in crude plant extracts.enCopyright © 2010, Springer Science+Business Media, LLCGlycochipMicroarrayOligosaccharidesPlant cell wallTransglycosylationXETJournal article002009411810.1007/s10719-009-9271-80002740371000082-s2.0-7794927214136529Hrmova, M. [0000-0002-3545-0605]