Warren-Smith, S.Nie, G.Schartner, E.Salamonsen, L.Monro, T.2013-01-102013-01-102012Biomedical Optics Express, 2012; 3(12):1-102156-70852156-7085http://hdl.handle.net/2440/74884A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignment. The auto-alignment system provides optical stability for the time required to perform an activity assay. The chosen assay is based on the enzyme proprotein convertase 5/6 (PC6) and has important applications in women's health.en© 2012 OSA(060.2370) Fiber optics sensors(060.4005) Microstructured fibers(280.1415) Biological sensing and sensorsJournal article00201237522013011013431810.1364/BOE.3.00330402 Physical Sciences0205 Optical Physics020599 Optical Physics not elsewhere classified0003127095000232-s2.0-8487075697522140Warren-Smith, S. [0000-0002-2612-6344]Schartner, E. [0000-0003-1669-4302]