Weng, Y.Yan, P.Sun, B.Wan, A.You, J.Xu, X.Lu, Z.Stewart, G.A.Chen, X.Song, H.Zhao, C.X.2025-01-062025-01-062024Chemical Engineering Journal, 2024; 497(1):154471-1-154471-101385-89471385-8947https://hdl.handle.net/2440/143485Enzyme immobilization within metal–organic frameworks (MOFs) addresses the inherent fragility of enzymes, playing a crucial role across diverse industries by improving efficiency and lowering economic costs. While the application of MOFs in the food and pharmaceutical industries is constrained by toxicity concerns, MIL-88A(Fe) emerges as an ideal candidate due to its non-toxicity and biocompatibility. However, the release of encapsulated enzymes is significantly hampered, reducing their bioactivity. Herein, we present a safe and simple platform for creating enzyme@MIL-88A, which provides enzyme stabilization and controlled release. The thermal stabilization of a spectrum of enzymes (phytase, xylanase, amylase, mannanase, and glucanase) is achieved, elevating their endurance threshold to 95 ◦C. Furthermore, the controlled on-demand release of the encapsulated enzymes at target sites is accomplished by adjusting defects in enzyme@MIL-88A composites via an acid modulation approach, while preserving enzyme activity. This approach has improved the amount of enzyme released from 10 % to 99.7 %. To the best of our knowledge, this is the first time enzyme@MIL-88A has been synthesized rapidly under mild conditions for enzyme stabilization and controlled release. Our method offers a universal platform for stabilizing vulnerable biomaterials and the controlled delivery of biological macromolecules.en© 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Metal–Organic framework; Enzyme encapsulation; Defect engineering; Controlled release; Thermal stabilityJournal article10.1016/j.cej.2024.154471703411