Hoang, N.Landolfi, A.Kravchuk, A.Girard, E.Peate, J.Hernandez, J.Gaborieau, M.Kravchuk, O.Gilbert, R.Guillaneuf, Y.Castignolles, P.2012-01-102012-01-102008Journal of Chromatography A, 2008; 1205(1-2):60-700021-96731873-3778http://hdl.handle.net/2440/69190The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 degrees C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.enCrown Copyright © 2008 Published by Elsevier B.V. All rights reserved.Size-exclusion chromatography (SEC)SeparationRepeatabilityLabelingUV detectionStarchJournal article002011446010.1016/j.chroma.2008.07.0900002598847000072-s2.0-5084913220626957Kravchuk, A. [0000-0003-1144-1285]Kravchuk, O. [0000-0001-5291-3600]