Mattiske, T.Tan, M.Gecz, J.Shoubridge, C.Hatters, D.Hannan, A.2013-11-252013-11-252013Tandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), vol.1017, Ch.9, pp.121-1339781627034371http://hdl.handle.net/2440/81224ISSN 1064-3745 (print) ISSN 1940-6029 (electronic)Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.en© Springer Science+Business Media New York 2013Polyalanine tract; ARX; Co-immunoprecipitation; Protein–protein interactionChallenges of 'sticky' co-immunoprecipitation: Polyalanine tract protein-protein interactionsBook chapter002013133010.1007/978-1-62703-438-8_92-s2.0-8488315858218338Mattiske, T. [0000-0002-9581-5370]Gecz, J. [0000-0002-7884-6861]Shoubridge, C. [0000-0002-0157-3084]