Brand, C.Ferrante, A.Prager, R.Riley, T.Carson, C.Finlay-Jones, J.Hart, P.2006-06-232006-06-232001Inflammation Research, 2001; 50(4):213-2191023-38301420-908Xhttp://hdl.handle.net/2440/7403<h4>Objective</h4>To evaluate the regulatory properties of the essential oil of Melaleuca alternifolia (tea tree oil) on the production of oxygen derived reactive species by human peripheral blood leukocytes activated in vitro.<h4>Materials and methods</h4>The ability of tea tree oil to reduce superoxide production by neutrophils and monocytes stimulated with N-formyl-methionyl-leucyl-phenylalanine (fMLP), lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA) was examined.<h4>Results</h4>The water-soluble fraction of tea tree oil had no significant effect on agonist-stimulated superoxide production by neutrophils, but significantly and dose-dependently suppressed agonist-stimulated superoxide production by monocytes. This suppression was not due to cell death. Chemical analysis identified the water-soluble components to be terpinen-4-ol, alpha-terpineol and 1,8-cineole. When examined individually, terpinen-4-ol significantly suppressed fMLP- and LPS- but not PMA-stimulated superoxide production; alpha-terpineol significantly suppressed fMLP-, LPS- and PMA-stimulated superoxide production; 1,8-cineole was without effect.<h4>Conclusion</h4>Tea tree oil components suppress the production of superoxide by monocytes, but not neutrophils, suggesting the potential for selective regulation of cell types by these components during inflammation.enNeutrophilsMonocytesCells, CulturedHumansSuperoxidesWaterCyclohexanolsMentholTerpenesTetradecanoylphorbol AcetateMonoterpenesLipopolysaccharidesTea Tree OilN-Formylmethionine Leucyl-PhenylalanineAnti-Infective Agents, LocalSolubilityCyclohexenesEucalyptolCyclohexane MonoterpenesJournal article002001092010.1007/s0001100507460001687033000042-s2.0-003503880861702Ferrante, A. [0000-0002-2581-6407]