Coates, Nicholas John Hewlett2006-12-282006-12-2819961996http://hdl.handle.net/2440/18950Errata sheet pasted opposite Table of Contents.Includes bibliographies.142, [171] leaves : ill. ; 30 cm.This thesis investigates the UV-induced inhibition of oriC initiation function and the nature of the phenotype of the mutant origin of replication of Escherichia coli, oriCc. The specific aims of this study are to delineate the source of the UV-induced trans-acting inhibition of oriC function, utilizing the phage vector [lambda]poriCc, and to demonstrate the oriCc allele as an enhanced DNA replication initiator from oriC.354662 bytesapplication/pdfenEscherichia coli Genetics.Transduction.DNA replication.Bacteriophage lambda.Mutagenesis.Ultraviolet radiation.Thesis