Mather, Diane ElizabethNguyen, Phuoc Xuan2018-06-072018-06-072016http://hdl.handle.net/2440/112722Title page, abstract and table of contents only. The complete thesis in print form is available from the University of Adelaide Library.Langdon 7D(7A) and 7D(7B) durum substitution lines were crossed with DBA-Aurora durum wheat to introgress a lutein esterification gene,TaGelp1, from chromosome 7D onto its homoeologues 7A and 7B. Genotyping-by-sequencing based on DNA samples from durum wheat and bread wheat revealed single nucleotide polymorphism (SNPs) among the group-7 chromosomes. Sixteen KASP markers were developed and to be able to differentiate among these chromosomes. Nine 7A-7D markers were used to characterise progeny populations to search for dissociation of molecular markers which may indicate chromosomal recombination. Evidence of possible 7A-7D recombination was found in a small number of progeny (less than 4%). Most of the putative marker dissociations were in the centromeric region but one plant was found to carry only a small distal fragment of 7DS including TaGelp1. The findings suggest crossing normal durum with Langdon 7D(7A) combined with KASP marker assistance can be applied as a method to introgress and assess genes from chromosome 7D onto its homoeologues without resorting to use of wheat with the Ph1 deletion.courseworkTriticum turgidum subsp. durumlutein esterificationgenotyping-by-sequencingKASP markerhomoeologous recombinationTheses