Harrison, Sharon Jane2006-12-282006-12-2819991999http://hdl.handle.net/2440/19384Bibliography: leaves 120-138.xi, 138, [41] leaves : ill. ; 30 cm.Characterises the 186 coliphage site-specific integration reaction in vitro with the expectation that it could be used in mammalian systems to insert genes or modify existing ones, to provide an alternative method for the production of transgenic livestock species. The analyzed system is the temperate bacteriophage 186. The in-vitro requirements for 186 integrative site-specific recombination were investigated. In vivo investigations were conducted whereby active 186-intasomes were microinjected into fertilised mouse eggs containing genomic copies of 186-attB. The 186 system has not been shown to work in vivo.297606 bytesapplication/pdfenTransgenic animalsBacteriophages Genetics.Thesis