Hunt, S.E.Netting, M.J.Sullivan, T.R.Best, K.P.Houghton, L.A.Makrides, M.Muhlhausler, B.S.Green, T.J.2025-07-172025-07-172020Nutrients, 2020; 12(5):1283-1-1238-62072-66432072-6643https://hdl.handle.net/2440/146112In 2009, the Australian government mandated the addition of folic acid to bread flour to reduce the incidence of neural tube defects (NTD)-affected pregnancies. In 2011–2012, the Australian Health Measures Survey (AHMS) reported a mean red blood cell (RBC) folate inwomenof reproductive age (16–44 y) of 1647 nmol/L. Over 99% of women had an RBC folate ≥ 906 nmol/L, a concentration consistent with a very low risk of NTDs if a woman became pregnant. However, RBC folate was measured using an immunoassay, which is not a recommended method due to questionable accuracy. The microbiological assay is the preferred method for RBC folate measurement. To determine whether the immunoassay method may have led to spurious conclusions about the folate status of Australian women, we collected fasting blood samples from 74 healthy non-pregnant, non-lactating women (18–44 y) and measured RBC folate using both the immunoassay and microbiological methods. Mean RBC folate (95% confidence interval) concentration measured with the immunoassay method was 1735 (1666, 1804) nmol/L compared with 942 (887, 1012) nmol/L using the microbiological method. No woman had an RBC folate < 906 nmol/L using the immunoassay method, whereas 46% of women had an RBC folate < 906 nmol/L using the microbiological method. The NTD risk was estimated to be 0.06% using the immunoassay method and 0.14% using the microbiological method. RBC folate using AHMS survey may have underestimated NTD risk in Australian women.en© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).red cell folate; microbiological assay; immunoassay; folate measurement; neural tube defectsErythrocytesHumansAnencephalyFolic AcidImmunoassayMicrobiological TechniquesHealth SurveysRiskDietary SupplementsAdolescentAdultAustraliaFemaleYoung AdultJournal article10.3390/nu120512832024-08-12530491Hunt, S.E. [0000-0003-4211-595X]Netting, M.J. [0000-0002-0642-9145]Sullivan, T.R. [0000-0002-6930-5406]Best, K.P. [0000-0002-7653-5074]Makrides, M. [0000-0003-3832-541X]Muhlhausler, B.S. [0000-0002-9021-6790]Green, T.J. [0000-0002-0667-4300]