Hao, N.Shearwin, K.E.2020-04-162020-04-162017Trends in Biochemical Sciences, 2017; 42(12):931-9320968-00041362-4326http://hdl.handle.net/2440/124218Bacterial sigma54 (σ⁵⁴) promoters are the DNA-binding motif for σ⁵⁴-containing RNA polymerase (RNAP) holoenzymes. A recent study using a combination of synthetic oligonucleotide library screening, biochemical characterization, and bioinformatics has uncovered a new and unexpected role for σ⁵⁴ promoters, encoding a form of bacterial 'insulator sequence' to dampen unwanted translation.en© 2017 Elsevier Ltd. All rights reserved.DNA-Directed RNA PolymerasesJournal article003007768810.1016/j.tibs.2017.10.0050004157960000012-s2.0-85032924922387585Hao, N. [0000-0001-5836-3507]Shearwin, K.E. [0000-0002-7736-2742]