Ebert, L.M.MacRaild, S.E.Davis, I.D.Cebon, J.Chen, W.2020-10-082020-10-082012Journal of Immunological Methods, 2012; 377(1-2):56-610022-17591872-7905http://hdl.handle.net/2440/128383Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.en© 2012 Elsevier B.V. All rights reserved.Fox P3, Antigen-specific regulatory T cells; CD3 down-regulation; NY-ESO-1Journal article100002672410.1016/j.jim.2012.01.0040003025010000082-s2.0-84862824595549285Ebert, L.M. [0000-0002-8041-9666]