Kant, K.Yoo, J.Amos, S.Erkelens, M.Priest, C.Shapter, J.Losic, D.2015-05-252015-05-252015RSC Advances: an international journal to further the chemical sciences, 2015; 5(30):23886-238912046-20692046-2069http://hdl.handle.net/2440/91317This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices.enJournal article003002544110.1039/c5ra01336d0003512358000882-s2.0-84926051797178808Losic, D. [0000-0002-1930-072X]