Gu, Y.Zhang, Y.Zhao, C.Pan, Y.Smales, R.Wang, H.Ni, Y.Zhang, H.Ni, J.Ma, J.Wang, L.2015-04-202015-04-202014Oral Diseases, 2014; 20(1):55-611354-523X1601-0825http://hdl.handle.net/2440/90489OBJECTIVES: The aim of the study was to determine whether the expression levels of specific circulating serum microRNAs (miRNAs) are associated with man- dibular prognathism (MP). METHODS: Sixty subjects in the early permanent denti- tion stage and 23 in the mixed dentition stage with MP were identified. Sixty-eight normal control subjects in the early permanent dentition stage and 24 in the mixed dentition stage were recruited for comparison. According to the microarray-based expression profiling, four serum miRNAs (let-7i-3p, miR-595, miR-16-2-3p, and miR-367-5p) were validated. RESULTS: In the MP groups, let-7i-3p was significantly over-expressed in subjects in the early permanent (P < 0.0005) and mixed (P < 0.001) dentitions, and miR- 595 was significantly under-expressed (P < 0.004) in sub- jects in the early permanent (P < 0.004) and mixed (P < 0.0005) dentitions, compared with normal control groups. Multiple logistic regression analysis and receiver operating characteristic curve analysis revealed that let- 7i-3p and miR-595 were able to significantly discrimi- nate MP subjects from normal controls. CONCLUSION: Let-7i-3p and miR-595 could be poten- tial, non-invasive biomarkers for the accurate early detection and diagnosis of MP, which may result in improved clinical management.en© 2013 John Wiley & Sonsmandibular prognathism; microRNA; serum; markerJournal article002013437710.1111/odi.120730003279595000072-s2.0-8489012824316561