Deng, F.Arman, A.Goldys, E.M.Hutchinson, M.R.Liu, G.2023-11-272023-11-272020ACS Applied Biomaterials, 2020; 3(1):539-5462576-64222576-6422https://hdl.handle.net/2440/139950Published online16 December 2019IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL–1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague–Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.en© 2019 American Chemical Societyspinal cord; cytokine sensing; IL-1β; rat intrathecal space; longitudinal experimentsJournal article10.1021/acsabm.9b009582023-11-23516714Hutchinson, M.R. [0000-0003-2154-5950]