Mrozik, K.Wada, N.Marino, V.Richter, W.Shi, S.Wheeler, D.Gronthos, S.Bartold, P.2013-12-092013-12-092013Regenerative Medicine, 2013; 8(6):711-7231746-07511746-076Xhttp://hdl.handle.net/2440/81470Aim: To investigate the capacity of allogeneic periodontal ligament stem cells (PDLSCs) to regenerate periodontal tissues using an ovine periodontal defect model. Materials & methods: Surgically created zero-wall dehiscence periodontal defects created in Merino sheep were filled with 1 × 107 allogeneic PDLSCs attached to Gelfoam®, Gelfoam alone or left untreated. After 4 weeks, histological analysis was performed to assess periodontal regeneration. Results: Allogeneic PDLSCs were well tolerated by recipient animals. The mean area of new alveolar bone was significantly greater in the PDLSC + Gelfoam treatment group compared with the defect-alone group. The PDLSC + Gelfoam and Gelfoam-only treatment groups displayed significantly greater length of new cementum and percentage of cementum regrowth compared with the defect-alone group. New Sharpey‘s fibers were generally more organized and significantly thicker within the PDLSC + Gelfoam treatment group. The PDLSC + Gelfoam treatment group also showed a trend of increased Sharpey‘s fiber attachment length compared with the Gelfoam-only and defect-alone groups. Conclusion: These studies support the potential use of allogeneic PDLSC preparations as viable therapies for periodontal regeneration in the clinical setting.en© Future Medicineallogeneicalveolar bonecementumperiodontal ligament stem cellsregenerationSharpey‘s fibersJournal article002013255310.2217/rme.13.660003263261000122-s2.0-8488658119017559Mrozik, K. [0000-0002-4890-8208]Gronthos, S. [0000-0002-6225-3084]Bartold, P. [0000-0002-5695-3877] [0000-0002-6225-3084]