Jusaitis, M.2013-05-292013-05-291997In Vitro Cellular and Developmental Biology: Plant, 1997; 33(3):213-2201054-54761475-2689http://hdl.handle.net/2440/78063Explants of axillary buds excised from mature adult stems of Swainsona formosa (G. Don) J. Thompson (syn. Clianthus formosus) were cultured on Mureshige and Skoog medium supplemented with a range of auxins, cytokinins, and sucrose concentrations. Auxins did not increase shoot or bud numbers above controls, and 2,4-dichlorophenoxyacetic acid was the only auxin to significantly increase callus production. Benzyladenine or thidiazuron incorporated into the medium at 0.1 μM stimulated shoot and bud production, and shoot growth occurred following removal of cytokinins from the medium after 4 wk. Shoot number increased linearly with sucrose concentration up to 40 g 1^-1, but shoot height and the number of cytokinin-induced buds were optimal at sucrose levels of 20-30 g 1^-1. Roots were initiated in vitro following treatment of cuttings with 0.1% indole-3-butyric acid and 0.1% α-naphthaleneacetic acid. Plantlets were successfully established in soil but were plagiotropic and exhibited distichous phyllotaxy.en© 1997 Society for In Vitro BiologyClianthus formosusGalegeaeLeguminosaePapilionoideaeplagiotropismtissue cultureJournal article00300005222013052313463310.1007/s11627-997-0025-7A1997XV920000112-s2.0-003141189664528Jusaitis, M. [0000-0002-0313-9636]