Westley, I.Coller, J.Ward, M.Evans, A.Morris, R.Sallustio, B.2016-11-032016-11-032014Journal of Liquid Chromatography and Related Technologies, 2014; 37(9):1249-12561082-60761520-572Xhttp://hdl.handle.net/2440/102144Background: A number of single nucleotide polymorphisms have been described in the ABCC2 gene that alters drug disposition. The aim of the study was to develop a primer extension denaturing high-performance liquid chromatography (PE-dHPLC) assay to determine three common variants of the ABCC2 gene in the promoter region (−24C>T), exon 10 (1249G>A), and exon 28 (3972C>T). Methods: Polymerase chain reactions (PCRs) were used to isolate the area of interest in the ABCC2 gene. A comparison of the PCR product was performed between sequencing and dHPLC. Results: A 100% identity match was achieved between groups and allowed for a quick and accurate method to determine three single nucleotide polymorphisms in a single extension reaction. This assay is the first of its type to determine three ABCC2 variants by dHPLCenCopyright © Taylor & Francis Group, LLCABCC2; genotype; polymerase chain reaction; polymorphisms; PE-dHPLC; single nudeotide polymorphismJournal article003001221510.1080/10826076.2013.7897960003339889000052-s2.0-84896117958108436Coller, J. [0000-0002-8273-5048]Sallustio, B. [0000-0002-0186-3073]