Bader, C.Sorvina, A.Simpson, P.Wright, P.Stagni, S.Plush, S.Massi, M.Brooks, D.2017-06-012017-06-012016FEBS Letters, 2016; 590(18):3051-30600014-57931873-3468http://hdl.handle.net/2440/105614Live cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3 (1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER(™) , shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER(™) localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses.en© 2016 Federation of European Biochemical SocietiesEndoplasmic reticulum; live cell imaging; rheniumJournal article003006491310.1002/1873-3468.123650003848077000012-s2.0-84987600694262012Brooks, D. [0000-0001-9098-3626]