Kennedy, B.Mclaughlin, R.Kennedy, K.Chin, L.Curatolo, A.Tien, A.Latham, B.Saunders, C.Sampson, D.2017-06-232017-06-232014Biomedical Optics Express, 2014; 5(7):2113-21242156-70852156-7085http://hdl.handle.net/2440/106197We present optical coherence micro-elastography, an improved form of compression optical coherence elastography. We demonstrate the capacity of this technique to produce en face images, closely corresponding with histology, that reveal micro-scale mechanical contrast in human breast and lymph node tissues. We use phase-sensitive, three-dimensional optical coherence tomography (OCT) to probe the nanometer-to-micrometer-scale axial displacements in tissues induced by compressive loading. Optical coherence micro-elastography incorporates common-path interferometry, weighted averaging of the complex OCT signal and weighted least-squares regression. Using three-dimensional phase unwrapping, we have increased the maximum detectable strain eleven-fold over no unwrapping and the minimum detectable strain is 2.6 με. We demonstrate the potential of mechanical over optical contrast for visualizing micro-scale tissue structures in human breast cancer pathology and lymph node morphology.en© 2014 Optical Society of America(100.5088) Phase unwrapping(110.1650) Coherence imaging(110.4500) Optical coherence tomographyJournal article003005033310.1364/BOE.5.0021130003389291000102-s2.0-84903695372256192Mclaughlin, R. [0000-0001-6947-5061]