Brady, J.Mannering, S.Kireta, S.Coates, P.Proietto, A.Cowan, P.d'Apice, A.Lew, A.2010-03-262010-03-262009Immunology and Cell Biology, 2009; 87(5):413-4180818-96411440-1711http://hdl.handle.net/2440/57085© 2009 Australasian Society for Immunology Inc. All rights reserved.Using heterologous prime-boost (DNA immunization followed by immunization with transfected cells), we have generated depleting mouse anti-baboon CD2 monoclonal antibodies (mAb). These anti-CD2 mAb recognized a diverse range of primate CD2 from New World monkeys and Old World monkeys to humans and have potent immunosuppressive activity for human allo-MLR responses and anti-tetanus-toxoid recall responses. There was no upregulation of activation markers or release of cytokines when the mAb were incubated with human peripheral blood mononuclear cells. Using chimeric NOD-SCID IL2rγnull mice, the mAb were shown to deplete human and cynomolgus monkey T cells in vivo. These anti-CD2 mAb may therefore be important immunological tools in allo- and xenotransplantation.enCD2depletingDNAhumanized micemonoclonalprimateJournal article002009113310.1038/icb.2009.40002680679000092-s2.0-6814918270338557