Ma, J.Bellon, M.Wishart, J.Young, R.Blackshaw, L.Jones, K.Horowitz, M.Rayner, C.2009-11-172009-11-172009American Journal of Physiology - Gastrointestinal and Liver Physiology, 2009; 296(4):G735-G7390193-18571522-1547http://hdl.handle.net/2440/53444Copyright © 2009 by the American Physiological SocietyThe incretin hormones, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), play an important role in glucose homeostasis in both health and diabetes. In mice, sucralose, an artificial sweetener, stimulates GLP-1 release via sweet taste receptors on enteroendocrine cells. We studied blood glucose, plasma levels of insulin, GLP-1, and GIP, and gastric emptying (by a breath test) in 7 healthy humans after intragastric infusions of 1) 50 g sucrose in water to a total volume of 500 ml (approximately 290 mosmol/l), 2) 80 mg sucralose in 500 ml normal saline (approximately 300 mosmol/l, 0.4 mM sucralose), 3) 800 mg sucralose in 500 ml normal saline (approximately 300 mosmol/l, 4 mM sucralose), and 4) 500 ml normal saline (approximately 300 mosmol/l), all labeled with 150 mg 13C-acetate. Blood glucose increased only in response to sucrose (P<0.05). GLP-1, GIP, and insulin also increased after sucrose (P=0.0001) but not after either load of sucralose or saline. Gastric emptying of sucrose was slower than that of saline (t50: 87.4+/-4.1 min vs. 74.7+/-3.2 min, P<0.005), whereas there were no differences in t50 between sucralose 0.4 mM (73.7+/-3.1 min) or 4 mM (76.7+/-3.1 min) and saline. We conclude that sucralose, delivered by intragastric infusion, does not stimulate insulin, GLP-1, or GIP release or slow gastric emptying in healthy humans.enStomachHumansGastric Inhibitory PolypeptideInsulinBlood GlucoseSucroseSweetening AgentsSingle-Blind MethodGastric EmptyingDose-Response Relationship, DrugTime FactorsAdultFemaleMaleGlucagon-Like Peptide 1IncretinsYoung AdultJournal article002009040810.1152/ajpgi.90708.20080002647064000062-s2.0-6714912186739112Young, R. [0000-0001-5116-4951] [0009-0004-8274-9863]Blackshaw, L. [0000-0003-1565-0850]Jones, K. [0000-0002-1155-5816]Horowitz, M. [0000-0002-0942-0306]Rayner, C. [0000-0002-5527-256X]