Fung, K.Y.C.Cursaro, C.Lewanowitsch, T.Cosgrove, L.Hoffmann, P.Kurien, B.J.Scofield, R.H.2015-05-172015-05-172012Methods in Molecular Biology, 2012; 869:135-14697816177982141064-37451940-6029http://hdl.handle.net/2440/91119Free flow electrophoresis (FFE) has been applied in numerous studies as a protein separation technique due to its multiple advantages such as fast and efficient sample recovery, high resolving power, high reproducibility, and wide applicability to protein classes. As a stand-alone platform however, its utility in comparative proteomic analysis is limited as protein samples must be run sequentially rather than simultaneously which introduces inherent variability when attempting to perform quantitative analysis. Here we describe an approach combining fluorescent CyDye technology (DIGE) with FFE to simultaneously separate and identify differentially expressed proteins in a model cell system.en© Springer Science+Business Media B.V. 2012Free flow electrophoresisFFEDIGEProteomicsJournal article003000955710.1007/978-1-61779-821-4_122-s2.0-8486583192085315