Adams, L.Marsh, J.Ayonrinde, O.Olynyk, J.Ang, W.Beilin, L.Mori, T.Palmer, L.Oddy, W.Lye, S.Pennell, C.2014-10-192014-10-192012Journal of Gastroenterology and Hepatology, 2012; 27(9):1520-15270815-93191440-1746http://hdl.handle.net/2440/86364<h4>Background and aim</h4>Environmental factors including excessive caloric intake lead to disordered lipid metabolism and fatty liver disease (FLD). However, FLD demonstrates heritability suggesting genetic factors are also important. We aimed to use a candidate gene approach to examine the association between FLD and single nucleotide polymorphisms (SNPs) in lipid metabolism genes in the adolescent population-based Western Australian Pregnancy (Raine) Cohort.<h4>Methods</h4>A total 951 seventeen year-olds underwent hepatic ultrasound, anthropometric and biochemical characterization, DNA extraction and genotyping for 57 SNPs in seven lipid metabolism genes (ApoB100, ATGL, ABHD5, MTTP, CETP, SREBP-1c, PPARα). Associations were adjusted for metabolic factors and Bonferroni corrected.<h4>Results</h4>The prevalence of FLD was 16.2% (11.4% male vs 21.2% female, P=0.001). Multivariate analysis of metabolic factors found suprailiac skinfold thickness (SST) to be the major predictor of FLD in females and males (odds ratio [OR] 1.11, 95% confidence interval [CI] 1.08-1.15, P=1.7×10(-10) and OR 1.17, 95%CI 1.13-1.22, P=2.4×10(-11) , respectively). In females, two SNPs in linkage disequilibrium from the CETP gene were associated with FLD: rs12447924 (OR 2.16, 95%CI 1.42-3.32, P=0.0003) and rs12597002 (OR=2.22, 95%CI 1.46-3.41 P=0.0002). In lean homozygotes, the probability of FLD was over 30%, compared with 10-15% in lean heterozygotes and 3-5% in lean wild-types. However, these associations were modified by SST, such that for obese individuals, the probability of FLD was over 30% in all genotype groups.<h4>Conclusions</h4>Cholesteryl ester transfer protein gene polymorphisms are associated with an increased risk of FLD in adolescent females. The effect is independent of adiposity in homozygotes, thereby placing lean individuals at a significant risk of FLD.en© 2012 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty LtdAdolescents; non-alcoholic fatty liver disease; raine cohortJournal article002013660610.1111/j.1440-1746.2012.07120.x0003079651000192-s2.0-8486536913915549Palmer, L. [0000-0002-1628-3055]