Barden, A.Burke, V.Mas, E.Beilin, L.Puddey, I.Watts, G.Irish, A.Mori, T.2017-05-082017-05-082015Journal of Hypertension, 2015; 33(9):1947-19530263-63521473-5598http://hdl.handle.net/2440/104968Background: Metabolism of arachidonic acid by cytochrome P450 [omega]-hydroxylase leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE) that regulates vascular function, sodium homeostasis and blood pressure (BP). Supplementation with n-3 fatty acids is known to alter arachidonic acid metabolism and reduce the formation of the lipid peroxidation products F2-isoprostanes, but the effect of n-3 fatty acids on 20-HETE has not been studied. Method: We previously reported a significant effect of n-3 fatty acids but not coenzyme Q10 (CoQ) to reduce BP in a double-blind, placebo-controlled intervention, wherein patients with chronic kidney disease (CKD) were randomized to n-3 fatty acids (4 g), CoQ (200 mg), both supplements or control (4 g olive oil), daily for 8 weeks. This study examined the effect of n-3 fatty acids on plasma and urinary 20-HETE in the same study, as well as plasma and urinary F2-isoprostanes, and relate these to changes in BP. Results: Seventy-four patients completed the 8-week intervention. n-3 fatty acids but not CoQ significantly reduced plasma 20-HETE (P = 0.001) and F2-isoprostanes (P < 0.001). In regression models adjusted for BP at baseline, postintervention plasma 20-HETE was a significant predictor of the fall in SBP (P < 0.0001) and DBP (P < 0.0001) after n-3 fatty acids. Conclusion: This is the first report that n-3 fatty acid supplementation reduces plasma 20-HETE in humans and that this associates with reduced BP. These results provide a plausible mechanism for the reduction in BP observed in patients with CKD following n-3 fatty acid supplementation.enCopyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.20-hydroxyeicosatetraenoic acid; chronic kidney disease; F2-isoprostanes; n-3 fatty acid supplementationJournal article003005793110.1097/HJH.00000000000006210003684972000282-s2.0-84942570588277368Mas, E. [0000-0003-2848-9613]