Alaei, S.Knaupp, A.S.Lim, S.M.Chen, J.Holmes, M.L.Änkö, M.L.Nefzger, C.M.Polo, J.M.2021-12-012021-12-012016Stem Cell Research, 2016; 17(1):49-531873-50611876-7753https://hdl.handle.net/2440/133479Reprogrammable mouse models engineered to conditionally express Oct-4, Klf-4, Sox-2 and c-Myc (OKSM) have been instrumental in dissecting molecular events underpinning the generation of induced pluripotent stem cells. However, until now these models have been reported in the context of the m2 reverse tetracycline-controlled transactivator, which results in low reprogramming efficiency and consequently limits the number of reprogramming intermediates that can be isolated for downstream profiling. Here, we describe an improved OKSM mouse model in the context of the reverse tetracycline-controlled transactivator 3 with enhanced reprogramming efficiency (>9-fold) and increased numbers of reprogramming intermediate cells albeit with similar kinetics, which we believe will facilitate mechanistic studies of the reprogramming process.en© 2016 The Author(s). Published by Elsevier B.V.Cells, CulturedFibroblastsAnimalsMiceTeratomaTetracyclinesProto-Oncogene Proteins c-mycCell DifferentiationPlasmidsOctamer Transcription Factor-3Kruppel-Like Transcription FactorsTranscriptional ActivationSOXB1 Transcription FactorsInduced Pluripotent Stem CellsCellular ReprogrammingCells, CulturedFibroblastsAnimalsMiceTeratomaTetracyclinesProto-Oncogene Proteins c-mycCell DifferentiationPlasmidsOctamer Transcription Factor-3Kruppel-Like Transcription FactorsTranscriptional ActivationSOXB1 Transcription FactorsInduced Pluripotent Stem CellsCellular ReprogrammingJournal article10.1016/j.scr.2016.05.0082021-12-01594195Polo, J.M. [0000-0002-2531-778X]