New caerin antibiotic peptides from the skin secretion of the Dainty Green Tree Frog Litoria gracilenta. Identification using positive and negative ion electrospray mass spectrometry

Maclean, M.Brinkworth, C.Bilusich, D.Bowie, J.Doyle, J.Llewellyn, L.Tyler, M.2007-02-252007-02-252006Toxicon, 2006; 47(6):664-6750041-01011879-3150http://hdl.handle.net/2440/23548Copyright © 2006 Elsevier Ltd All rights reserved.The skin secretion of the Dainty Green Tree Frog Litoria gracilenta contains 16 peptides, which protect the animal from predators, both large and small. A combination of negative and positive ion electrospray mass spectrometry together with Lys-C enzymic digest and Edman sequencing identifies three new wide-spectrum caerin 1 antibiotics, namely Caerin 1.17 [GLFSVLGSVAKHLLPHVAPIIAEKL-NH2], Caerin 1.18 [GLFSVLGSVAKHLLPHVVPVIAEKL-NH2], and Caerin 1.19 [GLFKVLGSVAKHLLPHVAPIIAEKL-NH2], and a narrow spectrum antibiotic Caerin 3.5 [GLWEKVKEKANELVSGIVEGVK-NH2].enDainty Green Tree Frogskin secretioncaerin antibiotic peptidesNew caerin antibiotic peptides from the skin secretion of the Dainty Green Tree Frog Litoria gracilenta. Identification using positive and negative ion electrospray mass spectrometryJournal article002006067810.1016/j.toxicon.2006.01.0190002379120000062-s2.0-3364616146152806