Ward, H.Higgs, N.Blackmore, T.Sadlon, T.2014-01-242014-01-241997Immunology and Cell Biology, 1997; 75(5):508-5100818-96411440-1711http://hdl.handle.net/2440/81789The 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements, cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.enCopyright status unknownCloningComplementGene promoterJournal article00300002212014012408134010.1038/icb.1997.7911 Medical and Health Sciences1107 Immunology110704 Cellular ImmunologyA1997YJ566000132-s2.0-003070358464227Ward, H. [0000-0002-3831-1205]