Oberoi, A.Giezenaar, C.Rigda, R.S.Lange, K.Horowitz, M.Jones, K.L.Chapman, I.Soenen, S.2025-07-142025-07-142022Nutrients, 2022; 14(15):3111-1-3111-162072-66432072-6643https://hdl.handle.net/2440/145897The ingestion of dietary protein with, or before, carbohydrate may be a useful strategy to reduce postprandial hyperglycemia, but its effect in older people, who have an increased predisposition for type 2 diabetes, has not been clarified. Blood glucose, plasma insulin and glucagon concentrations were measured for 180 min following a drink containing either glucose (120 kcal), whey-protein (120 kcal), whey-protein plus glucose (240 kcal) or control (∼2 kcal) in healthy younger (n = 10, 29 ± 2 years; 26.1 ± 0.4 kg/m²) and older men (n = 10, 78 ± 2 years; 27.3 ± 1.4 kg/m²). Mixed model analysis was used. In both age groups the co-ingestion of protein with glucose (i) markedly reduced the increase in blood glucose concentrations following glucose ingestion alone (p < 0.001) and (ii) had a synergistic effect on the increase in insulin concentrations (p = 0.002). Peak insulin concentrations after protein were unaffected by ageing, whereas insulin levels after glucose were lower in older than younger men (p < 0.05) and peak insulin concentrations were higher after glucose than protein in younger (p < 0.001) but not older men. Glucagon concentrations were unaffected by age. We conclude that the ability of whey-protein to reduce carbohydrate-induced postprandial hyperglycemia is retained in older men and that protein supplementation may be a useful strategy in the prevention and management of type 2 diabetes in older people.en© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).whey protein; dietary glucose; blood glucose; insulin; glucagon; agingHumansDiabetes Mellitus, Type 2HyperglycemiaGlucagonInsulinGlucoseBlood GlucosePostprandial PeriodAgedMaleGlucagon-Like Peptide 1Whey ProteinsJournal article10.3390/nu141531112024-05-06617780Oberoi, A. [0000-0003-0517-8257]Lange, K. [0000-0003-3814-8513]Horowitz, M. [0000-0002-0942-0306]Jones, K.L. [0000-0002-1155-5816]Soenen, S. [0000-0002-0196-128X]