Radford, J.Wang, L.Li, J.Coad, B.McFarland, C.Nordon, R.2017-10-172017-10-172013Proceedings / IEEE International Symposium on Biomedical Imaging: from nano to macro. IEEE International Symposium on Biomedical Imaging, 2013, pp.1066-106997814673645461945-79281945-8452http://hdl.handle.net/2440/108641A novel algorithm, Adjacent Pixel Temporal Intensity Correlation (APTIC), was developed to detect single fluorescent molecules by their stochastic emission patterns; photoblinking and photobleaching. The algorithm was evaluated using simulated image data and Total Internal Reflection Fluorescence Microscopy (TIRF-M) to count the number fluorescently labelled protein molecules adsorbed onto glass substrates modified by Radio Frequency Glow Discharge (RFGD) deposition. By selecting an appropriate correlation threshold, the algorithm was capable of detecting synthetic flashes with a signal-to-noise ratio (SNR) as low as 2.0 with 90% sensitivity. The methodology holds great promise for mapping the amount and distribution of biomolecules on surfaces.en© 2013 IEEETIRF-M, single molecule imaging, protein adsorption, flash detectionConference paper003006275610.1109/ISBI.2013.65566620003269001002672-s2.0-84881646107283704Coad, B. [0000-0003-3297-3977]