Wang, H.Linyan Wu, L.Song, X.Luo, X.Zhong, J.Rush, R.Zhou, X.2010-01-122010-01-122006European Journal of Neuroscience, 2006; 24(9):2444-24520953-816X1460-9568http://hdl.handle.net/2440/55258Recent studies have shown that the precursor of brain-derived neurotrophic factor (pro-BDNF) activates p75NTR with high affinity to induce apoptosis. Here we show that pro-BDNF is transported anterogradely and retrogradely in sensory neurons of adult rats. After a crush injury of sciatic nerves, dorsal roots or dorsal column in adult Sprague–Dawley rats, the immunoreactivity for pro-BDNF accumulated at both the proximal and distal segments. The accumulation reached a maximum at 24 h after injury. Western blot analysis also revealed pro-BDNF in sciatic nerve segments proximal and distal to the ligature and in the spinal cord. Biotinylated or Alexa-488-labelled pro-BDNF injected into sciatic nerve was internalized and transported both retrogradely and anterogradely within sensory neurons. These results demonstrate that pro-BDNF is anterogradely and retrogradely transported in sensory neurons, suggesting that endogenous pro-BDNF may be released and play important functions.enSpinal CordNeurons, AfferentSciatic NerveSpinal Nerve RootsAnimalsMiceRatsRats, Sprague-DawleyBrain-Derived Neurotrophic FactorBlotting, WesternNerve CrushAxonal TransportJournal article002009424010.1111/j.1460-9568.2006.05138.x0002419535000032-s2.0-3375091220736461Zhou, X. [0000-0002-8687-0175]