Moore, E.Delalat, B.Vasani, R.McPhee, G.Thissen, H.Voelcker, N.2025-12-172025-12-172014ACS applied materials & interfaces, 2014; 6(17):15243-152521944-82441944-8252https://hdl.handle.net/1959.8/161277Data source: Supporting Information, http://pubs.acs.org/doi/suppl/10.1021/am503570vAnionic ring-opening polymerization of glycidol was initiated from activated glass, silicon, and porous silicon substrates to yield thin, ultralow-fouling hyperbranched polyglycerol (HPG) graft polymer coatings. Substrates were activated by deprotonation of surface-bound silanol functionalities. HPG polymerization was initiated upon the addition of freshly distilled glycidol to yield films in the nanometer thickness range. X-ray photoelectron spectroscopy, contact angle measurements, and ellipsometry were used to characterize the resulting coatings. The antifouling properties of HPG-coated surfaces were evaluated in terms of protein adsorption and the attachment of mammalian cells. The adsorption of bovine serum albumin and collagen type I was found to be reduced by as much as 97 and 91%, respectively, in comparison to untreated surfaces. Human glioblastoma and mouse fibroblast attachment was reduced by 99 and 98%, respectively. HPG-grafted substrates outperformed polyethylene glycol (PEG) grafted substrates of comparable thickness under the same incubation conditions. Our results demonstrate the effectiveness of antifouling HPG graft polymer coatings on a selected range of substrate materials and open the door for their use in biomedical applications.enCopyright 2014 American Chemical Societyhyperbranched polyglycerolsurface graftingbiofoulingantifoulinglow-foulingnonfoulingJournal article10.1021/am503570v2-s2.0-84907829548