Kelly, J.Ward, H.Miles, M.Kendall, G.2014-01-272014-01-271992Nucleic Acids Research, 1992; 20(15):3963-39690305-10481362-4962http://hdl.handle.net/2440/81798A Trypanosoma cruzi expression vector has been constructed using sequences derived from the flanking regions of the glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) genes. The neomycln phosphotransferase (neor) gene was incorporated as a selectable marker. Using electroporatlon we have introduced this vector into both T.cruzl and Leishmania cells and conferred G418 resistance. Transformation is mediated by large extrachromosomal circular elements composed of head-to-tail tandem repeats of the vector. The transformed phenotype is stable for at least 6 months in the absence of G418 and can be maintained during passage through the T.cruzl ifecycle. Foreign genes Inserted into an expression site within the vector (pTEX) can be expressed at high levels In transformed cells. To our knowledge this paper describes the first trypanosome shuttle vector and the first vector which functions in both trypanosomes and Leishmania.en© 1992 Oxford University PressAnimalsLeishmania donovaniLeishmania mexicanaTrypanosoma cruziGlyceraldehyde-3-Phosphate DehydrogenasesPhosphotransferasesKanamycin KinaseRecombinant Fusion ProteinsBlotting, SouthernCloning, MolecularTransfectionGene ExpressionBase SequenceGenetic VectorsPlasmidsMolecular Sequence DataJournal article00300002152014012807524810.1093/nar/20.15.396306 Biological Sciences0605 Microbiology060502 Infectious AgentsA1992JJ802000212-s2.0-002667361464221Ward, H. [0000-0002-3831-1205]