Elevated concentration and biologic activity of monocyte chemotactic protein-1 levels in the peritoneal fluid of patients with endometriosis

Akoum, A.Lemay, A.McColl, S.Turcot-Tremblay, L.Maheux, R.2006-07-052006-07-051996Fertility and Sterility, 1996; 66(1):17-230015-02821556-5653http://hdl.handle.net/2440/11561<h4>Objective</h4>To estimate the concentration and the biologic activity of monocyte chemotactic protein-1 (MCP-1) in the peritoneal fluid (PF) of women with and without endometriosis.<h4>Design</h4>A case control study was conducted.<h4>Setting</h4>Gynecology clinic and Laboratories of endocrinology of reproduction and immunology.<h4>Patients</h4>Women presenting for infertility, pelvic pain, or tubal ligation in which endometriosis was diagnosed at laparoscopy (n = 36) and normal fertile controls presenting for tubal ligation (n = 21).<h4>Interventions</h4>Collection of PF via laparoscopy.<h4>Main outcome measures</h4>Determination of PF concentrations of MCP-1 by an ELISA and evaluation of its monocyte chemotactic activity using a human hystiocytic cell line (U937). RESULTS. The concentration of MCP-1 (median, range of values) was increased in the PF of endometriosis patients (283, 0 to 1,930 pg/mL; conversion factor to SI unit, 0.155) compared with the control group (140, 0 to 435 pg/mL). The most significant elevation of MCP-1 levels was found in the stage II of the disease (371, 200 to 1,930 pg/mL). An increased chemotactic activity for monocytes (mean number of migrating cells/mm2 +/- SD) also was found in stages I (1,460 +/- 312) and II (1,541 +/- 336) of the disease when compared with fertile controls (393 +/- 56). Forty percent to 53% of this activity was inhibited in the presence of an antibody specific to MCP-1.<h4>Conclusions</h4>These observations are consistent with previous data indicating increased leukocyte chemotaxis in the PF of patients with endometriosis and suggest that MCP-1 may play a relevant role in the peritoneal inflammatory reaction associated with the disease.enMonocytesAscitic FluidHumansEndometriosisCase-Control StudiesChemotaxis, LeukocyteOsmolar ConcentrationAdultFemaleChemokine CCL2Elevated concentration and biologic activity of monocyte chemotactic protein-1 levels in the peritoneal fluid of patients with endometriosisJournal article0030004235001996564110.1016/s0015-0282(16)58381-22-s2.0-002995079768241McColl, S. [0000-0003-0949-4660]