Das, A.Ajuebor, M.Flower, R.Perretti, M.McColl, S.2006-07-052006-07-051999Clinical and Experimental Immunology, 1999; 117(2):223-2290009-91041365-2249http://hdl.handle.net/2440/11548Cell accumulation and CC chemokine production were assessed in the peritoneal cavity of ovalbumin (OVA)-sensitized mice following antigen challenge. Intraperitoneal challenge with OVA induced a significant eosinophil influx from 6 h post-challenge with increased numbers persisting at 24 h. At 6 h there was also a marked presence of neutrophils. Messenger RNA expression and protein levels for the chemokines RANTES and MIP-1 alpha were measured in the cell pellets and supernatants, respectively, from peritoneal washes following OVA challenge. RANTES mRNA was detected from 2 h to 4 h following OVA injection, whereas mRNA for MIP-1 alpha was only detectable at 4 h. RANTES protein was first detected from 4 h after OVA injection and by 24 h the protein levels had increased further. Basal levels of MIP-1 alpha were detected in peritoneal washes. These levels peaked at 2 h after OVA challenge and rapidly declined to basal levels by 6 h. A functional role for the chemokines was assessed using neutralizing polyclonal antibodies. Co-injection of OVA with anti-RANTES antibodies resulted in a significant inhibition of eosinophil infiltration into the cavity at 6 h and 24 h (63% and 52% inhibition, respectively) without significantly influencing the number of neutrophils present. In contrast, injection of anti-MIP-1 alpha antibodies only inhibited neutrophil migration at the 6 h time point by 44% without significantly affecting the accumulation of eosinophils. These results demonstrate an important role for RANTES in mediating eosinophil influx in allergic inflammation and a contrasting role for MIP-1 alpha in mediating neutrophil recruitment.enPeritoneal CavityEosinophilsNeutrophilsAnimalsMice, Inbred BALB CMicePeritonitisHypersensitivityDisease Models, AnimalOvalbuminMacrophage Inflammatory ProteinsImmune SeraInjections, IntraperitonealCell MovementTime FactorsFemaleChemokine CCL5Chemokine CCL4Journal article0030004245001999039610.1046/j.1365-2249.1999.00978.x0000826776000042-s2.0-003281765168251McColl, S. [0000-0003-0949-4660]