Watts-Fawkes, S.J.Jewell, N.Brien, C.Berger, B.Garnett, T.Cavagnaro, T.R.2021-12-142021-12-142019Plant Phenomics, 2019; 2019:5893953-1-5893953-122643-65152643-6515https://hdl.handle.net/2440/133683There are many reported benefits to plants of arbuscular mycorrhizal fungi (AMF), including positive plant biomass responses; however, AMF can also induce biomass depressions in plants, and this response receives little attention in the literature. High-throughput phenotyping (HTP) technology permits repeated measures of an individual plant’s aboveground biomass. We examined the effect on AMF inoculation on the shoot biomass of three contrasting plant species: a vegetable crop (tomato), a cereal crop (barley), and a pasture legume (Medicago).We also considered the interaction of mycorrhizal growth responses with plant-available soil zinc (Zn) and phosphorus (P) concentrations. Te appearance of a depression in shoot biomass due to inoculation with AMF occurred at different times for each plant species; depressions appeared earliest in tomato, then Medicago, and then barley. The usually positive-responding Medicago plants were not responsive at the high level of soil available P used. Mycorrhizal growth responsiveness in all three species was also highly interactive with soil Zn supply; tomato growth responded negatively to AMF inoculation in all soil Zn treatments except the toxic soil Zn treatment, where it responded positively. Our results illustrate how context-dependent mycorrhizal growth responses are and the value of HTP approaches to exploring the complexity of mycorrhizal responses.enCopyright © 2019 S. J. Watts-Williams et al. Exclusive Licensee Nanjing Agricultural University. Distributed under a Creative Commons Attribution License (CC BY 4.0).Using High-Throughput Phenotyping to Explore Growth Responses to Mycorrhizal Fungi and Zinc in Three Plant SpeciesJournal article10.34133/2019/58939532021-12-14554183Watts-Fawkes, S.J. [0000-0003-3467-0662]Brien, C. [0000-0003-0581-1817]Berger, B. [0000-0003-1195-4478]Garnett, T. [0000-0003-1664-9659]Cavagnaro, T.R. [0000-0002-9922-5677]