Torok, V. A.Randles, John Wesley2007-05-112007-05-112001Australasian Plant Pathology, 2001; 30 (3):227-2300815-3191http://hdl.handle.net/2440/27732Tobacco mosaic virus (TMV) is an easily prepared, plentiful and stable source of homogenous RNA suitable for addition to reverse transcription polymerase chain reaction (RT-PCR) assays as an internal control. We describe primers and conditions used in a duplex assay for Pea seedborne mosaic virus (PSbMV)-infection of pea seed as an example of the utility of the system for RT-PCR screening of plant germplasm for the presence or absence of a virus.enPisum sativum; Potyvirus; PSbMV diagnostic RT-PCR primers; TMV RT-PCR primersJournal article002001003810.1071/AP01028