Ling, K.Brautigan, P.Moore, S.Fraser, R.Leong, M.Leong, J.Zainal Abidin, S.Lee, H.Cheah, P.Raison, J.Babic, M.Lee, Y.Daish, T.Mattiske, D.Mann, J.Adelson, D.Thomas, P.Hahn, C.Scott, H.2017-08-102017-08-102016Data in Brief, 2016; 7:282-2902352-34092352-3409http://hdl.handle.net/2440/106762Available online 17 February 2016SRY (Sex Determining Region Y)-Box 4 or Sox4 is an important regulator of the pan-neuronal gene expression during post-mitotic cell differentiation within the mammalian brain. Sox4 gene locus has been previously characterized with multiple sense and overlapping natural antisense transcripts [1], [2]. Here we provide accompanying data on various analyses performed and described in Ling et al. [2]. The data include a detail description of various features found at Sox4 gene locus, additional experimental data derived from RNA-Fluorescence in situ Hybridization (RNA-FISH), Western blotting, strand-specific reverse-transcription quantitative polymerase chain reaction (RT-qPCR), gain-of-function and in situ hybridization (ISH) experiments. All the additional data provided here support the existence of an endogenous small interfering- or PIWI interacting-like small RNA known as Sox4_sir3, which origin was found within the overlapping region consisting of a sense and a natural antisense transcript known as Sox4ot1.en© 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Endogenous siRNABrain developmentNatural antisense transcriptsJournal article003004493510.1016/j.dib.2016.01.0452-s2.0-84958064612238310Adelson, D. [0000-0003-2404-5636]Hahn, C. [0000-0001-5105-2554]Scott, H. [0000-0002-5813-631X]