Selth, L.Close, T.Svejstrup, J.Tollefsbol, T.2012-06-202012-06-202011Epigenetics Protocols, 2011 / Tollefsbol, T. (ed./s), vol.791, pp.253-2649781617793158http://hdl.handle.net/2440/71701The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.enRNA immunoprecipitationRNA-binding proteintranscription factormRNAchromatin immunoprecipitationgene expressionBook chapter002011493710.1007/978-1-61779-316-5_192-s2.0-800547503326704Selth, L. [0000-0002-4686-1418]