Doherty, D.Z.Ghith, A.Ho, A.De Voss, J.J.Bell, S.G.2023-08-072023-08-072023Chemical Communications, 2023; 59(61):9392-93951359-73451364-548Xhttps://hdl.handle.net/2440/139080Cholesterol catabolism is an important survival mechanism for the pathogenic Mycobacterium tuberculosis. Various other mycobacteria degrade not only cholesterol but plant sterols such as sitosterol and campesterol. In this work we demonstrate that the cytochrome P450 (CYP) CYP125 enzyme family is capable of sitosterol and campesterol side-chain oxidation and activation in these bacteria. We also show that the CYP142 and CYP124 cholesterol hydroxylating enzyme families are significantly less active for sitosterol hydroxylation compared to CYP125 enzymes.en© The Royal Society of Chemistry 2023.Mycobacterium tuberculosisCholesterolSitosterolsCytochrome P-450 Enzyme SystemOxidation-ReductionMycobacterium tuberculosisCholesterolSitosterolsCytochrome P-450 Enzyme SystemOxidation-ReductionJournal article10.1039/d3cc02312e2023-08-07649905Ghith, A. [0000-0003-1753-6939]Bell, S.G. [0000-0002-7457-9727]