Curtin, C.Borneman, A.Chambers, P.Pretorius, I.Fairhead, C.2013-05-072013-05-072012PLoS One, 2012; 7(3):1-101932-62031932-6203http://hdl.handle.net/2440/77480Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits.en© 2012 Curtin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Sequence Analysis, DNAPhylogenyHaplotypesPolymorphism, Single NucleotideGenome, FungalOpen Reading FramesWineDekkeraJournal article002012708310.1371/journal.pone.00338400003044890000382-s2.0-8485902189220314Borneman, A. [0000-0001-8491-7235]