Li, S.Wilkinson, K.L.Mierczynska-Vasilev, A.Bindon, K.A.2021-08-252021-08-252019Molecules, 2019; 24(11):2100-1-2100-181420-30491420-3049http://hdl.handle.net/2440/131603Published: 3 June 2019Interactions between grape seed tannin and either a mannoprotein or an arabinogalactan in model wine solutions of different ethanol concentrations were characterized with nanoparticle tracking analysis (NTA), UV-visible spectroscopy and dynamic light scattering (DLS). NTA results reflected a shift in particle size distribution due to aggregation. Furthermore, the light scattering intensity of each tracked particle measured by NTA demonstrated the presence of aggregates, even when a shift in particle size was not apparent. Mannoprotein and arabinogalactan behaved differently when combined with seed tannin. Mannoprotein formed large, highly light-scattering aggregates, while arabinogalactan exhibited only weak interactions with seed tannin. A 3% difference in alcohol concentration of the model solution (12 vs. 15% v/v) was sufficient to affect the interactions between mannoprotein and tannin when the tannin concentration was high. In summary, this study showed that NTA is a promising tool for measuring polydisperse samples of grape and wine macromolecules, and their aggregates under wine-like conditions. The implications for wine colloidal properties are discussed based on these results.en© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).mannoproteinarabinogalactanseed tanninaggregationnanoparticle tracking analysisJournal article003011804510.3390/molecules241121000004726310000732-s2.0-85066745252476810Li, S. [0000-0002-0246-8948]Wilkinson, K.L. [0000-0001-6724-9837]