Lawrenson, J.Ghabriel, M.Reid, A.Gajree, T.Allt, G.2006-06-232006-06-231995British Journal of Ophthalmology, 1995; 79(5):462-4660007-11611468-2079http://hdl.handle.net/2440/5339BACKGROUND--A rat endothelial barrier antigen (EBA) recognised by a monoclonal antibody has been shown to be expressed strongly by endothelial cells of brain capillaries possessing a blood-brain barrier and only weakly expressed by fenestrated brain vessels. METHODS--In this study immunocytochemical methods for light and electron microscopy were used to study EBA distribution in the eye and orbital tissues of the rat. RESULTS--Blood-ocular barrier vessels in the optic nerve, retina, iris, and some vessels in th choroid and ciliary body were immunopositive for EBA. By pre-embedding immunocytochemistry for electron microscopy the antigen was observed on the luminal endothelial cell surface. CONCLUSION--Surprisingly, some non-barrier vessels in the ciliary body and choroid expressed EBA suggesting that it may play a broader role in endothelial properties than previously recognised. The functional significance of EBA remains to be elucidated.enEyeOrbitBlood-Brain BarrierOptic NerveRetinaChoroidCiliary BodyIrisAnimalsRatsRats, Sprague-DawleyAntigens, SurfaceMicroscopy, ImmunoelectronImmunohistochemistryMaleJournal article0030006268001995048310.1136/bjo.79.5.462A1995RA260000152-s2.0-002906005770274Ghabriel, M. [0000-0002-9153-271X]