Chong, J.J.H.Chandrakanthan, V.Xaymardan, M.Asli, N.S.Li, J.Ahmed, I.Heffernan, C.Menon, M.K.Scarlett, C.J.Rashidianfar, A.Biben, C.Zoellner, H.Colvin, E.K.Pimanda, J.E.Biankin, A.V.Zhou, B.Pu, W.T.Prall, O.W.J.Harvey, R.P.2025-07-152025-07-152011Cell Stem Cell, 2011; 9(6):527-5401934-59091875-9777https://hdl.handle.net/2440/145940Colony-forming units – fibroblast (CFU-Fs), analogous to those giving rise to bone marrow (BM) mesenchymal stem cells (MSCs), are present in many organs, although the relationship between BM and organspecific CFU-Fs in homeostasis and tissue repair is unknown. Here we describe a population of adult cardiac-residentCFU-Fs (cCFU-Fs) that occupy a perivascular, adventitial niche and show broad trans-germ layer potency in vitro and in vivo. CRE lineage tracing and embryo analysis demonstrated a proepicardial origin for cCFU-Fs. Furthermore, inBMtransplantation chimeras, we found no interchange between BM and cCFU-Fs after aging, myocardial infarction, or BM stem cell mobilization. BM and cardiac and aortic CFU-Fs had distinctCRE lineage signatures, indicating that they arise from different progenitor beds during development. These diverse origins for CFU-Fs suggest an underlying basis for differentiation biases seen in different CFU-F populations, and could also influence their capacity for participating in tissue repair.en© 2011 Elsevier Inc.Colony-forming units – fibroblast (CFU-Fs); bone marrow (BM); mesenchymal stem cells (MSCs); organs; homeostasis and tissue repair; adults; cardiac-residentCFU-Fs (cCFU-Fs); embryo; in vitro and in vivoHeartPericardiumBone Marrow CellsCells, CulturedFibroblastsMyocytes, CardiacMesenchymal Stem CellsAnimalsTransplantation ChimeraMiceColony-Forming Units AssayCell DifferentiationCell LineageBiomarkersJournal article10.1016/j.stem.2011.10.0022024-05-12632224Chandrakanthan, V. [0000-0002-4314-5029]