Nair, R.Habili, N.Randles, J.2010-06-022010-06-022009Australasian Plant Disease Notes, 2009; 4(1):46-481833-928Xhttp://hdl.handle.net/2440/58633Severe yellow mosaic and leaf distortion symptoms have been observed on Cullen australasicum (cullen) plants growing in experimental plots at Urrbrae, South Australia. The incidence of the affected plants in a 2-year-old stand was ~80%. Symptom distribution on plants was uneven and symptom intensity appeared to vary between seasons. Alfalfa mosaic virus (AMV) was identified in 6/10 symptomatic and 1/10 asymptomatic field plants by reverse transcription-polymerase chain reaction (RT–PCR). Transmission of mosaic symptoms from cullen to cullen by mechanical inoculation was unsuccessful, but a cullen AMV isolate passaged through herbaceous indicators induced systemic mosaic in 1/4 of inoculated cullen seedlings. No seed transmission of AMV was observed in 50 seedlings raised from symptomatic parents. These results show that cullen is a host of AMV, but further studies are needed to determine whether AMV is the sole cause of the mosaic disease.enCopyright Australasian Plant Pathology Society 2009Journal article002009757710.1071/DN090192-s2.0-7104919549434296Habili, N. [0000-0001-5969-9547]Randles, J. [0000-0003-2231-816X]