Fung, K.Cursaro, V.Lewanowitsch, T.Brierley, G.McColl, S.Lockett, T.Head, R.Hoffmann, P.Cosgrove, L.2011-09-122011-09-122011Proteomics, 2011; 11(5):964-9711615-98531615-9861http://hdl.handle.net/2440/66076Article first published online: 31 JAN 2011Many biologically active agents exert a pleiotropic response in cells and tissues. This presents challenges in descriptive and comparative analysis of the proteome in response to these agents. Although free-flow electrophoresis has been applied in a number of proteomic studies as a protein separation technique, the combination of free-flow electrophoresis and DIGE has not yet been investigated for comparative proteomic analysis. In this study, we have compared the effects of butyrate on HT29 colorectal cancer cells with a particular focus on apoptosis and describe the utility of a novel approach combining free-flow electrophoresis with DIGE to identify differentially expressed proteins. We verify the results obtained by the combined free-flow electrophoresis and DIGE approach with Western blot analysis of selected proteins. We also report for the first time the regulation of a number of proteins by butyrate in HT29 colorectal cells including peptidyl-prolyl cis-trans isomerase A (cyclophilin A) and profilin-1.enCopyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimButyrateColorectal cancerDIGEFree-flow electrophoresisTechnologyJournal article002011076610.1002/pmic.2010004290002881373000122-s2.0-7995173488728706McColl, S. [0000-0003-0949-4660]Head, R. [0000-0002-1196-0926]