Smith, K.Hale, J.Austin, J.Melville, J.2011-11-032011-11-032011Conservation Genetics Resources, 2011; 3(4):621-6241877-72521877-7260http://hdl.handle.net/2440/67160Eight polymorphic microsatellite loci were identified in the Australian tree frog, Litoria ewingii, for studying patterns of hybridization with the closely related species L. paraewingi, using a microsatellite enriched genomic library. Across 55 individuals of L. ewingii and L. paraewingi, the mean number of alleles per locus was Na=9.38 and Na=7.13, respectively. All loci successfully cross-amplified in the remaining species in the Litoria ewingii complex, including the threatened sub-species L. verreauxii alpina. These loci will be useful in resolving species boundaries and exploring hybridization between species in the Litoria ewingii complex.en© Springer Science+Business Media B.V. 2011Hybrid zoneMicrosatelliteLitoria ewingiiLitoria paraewingiLitoria verreauxii alpinaAmphibianSouth-eastern AustraliaTree frogSpecies boundariesHybridizationIsolation and characterization of microsatellite markers for the Litoria ewingii complex and their use in conservation and hybridization studiesJournal article002011238210.1007/s12686-011-9418-30002946821000062-s2.0-8485987396727943Austin, J. [0000-0003-4244-2942]