Goldenberg, J.Cipriani, V.Jackson, T.N.W.Arbuckle, K.Debono, J.Dashevsky, D.Panagides, N.Ikonomopoulou, M.P.Koludarov, I.Li, B.Santana, R.C.Nouwens, A.Jones, A.Hay, C.Dunstan, N.Allen, L.Bush, B.Miles, J.J.Ge, L.Kwok, H.F.et al.2020-06-182020-06-182018Comparative Biochemistry and Physiology C: Toxicology and Pharmacology, 2018; 205:53-611532-04561878-1659http://hdl.handle.net/2440/126103Pseudechis (black snakes) is an Australasian elapid snake genus that inhabits much of mainland Australia, with two representatives confined to Papua New Guinea. The present study is the first to analyse the venom of all 9 described Pseudechis species (plus one undescribed species) to investigate the evolution of venom composition and functional activity. Proteomic results demonstrated that the typical Pseudechis venom profile is dominated by phospholipase A₂ toxins. Strong cytotoxicity was the dominant function for most species. P. porphyriacus, the most basal member of the genus, also exhibited the most divergent venom composition, being the only species with appreciable amounts of procoagulant toxins. The relatively high presence of factor Xa recovered in P. porphyriacus venom may be related to a predominantly amphibian diet. Results of this study provide important insights to guide future ecological and toxinological investigations.en© 2018 Elsevier Inc. All rights reserved.Venom evolution; Pseudechis; black snakes; diet; toxins; proteomic; enzymology; oxyuraninae; PLA₂Journal article100002083910.1016/j.cbpc.2018.01.001531819Cipriani, V. [0000-0002-4026-7725]